目的 建立朝药材肾炎草的HPLC-UV指纹图谱。方法 采用SunFireTM C18 (4.6 mm×250 mm, 5 μm)色谱柱;以乙腈(B)-0.4%冰醋酸溶液(A)梯度洗脱,检测波长253 nm,柱温40 ℃,进样量10 μL,流速1.0 mL · min-1,采用对照品指认进行成分分析。结果 建立了朝药材肾炎草的HPLC-UV特征指纹图谱共有模式,标定了21个共有峰,指认了其中3个黄酮碳苷成分。10批次药材的相似度结果均大于0.95,相似程度高。结论 该方法简便,精密度、重复性和稳定性很好,特征性和专属性强,可为肾炎草药材整体质量控制提供有效手段。
Abstract
OBJECTIVE To establish the fingerprint of Melandrium firmum Rohrb. by HPLC-UV. METHODS The analysis was performed on SunFireTM C18 (4.6 mm×250 mm, 5 μm) column. The mobile phase was acetonitrile (B) and 0.4% phosphoric acid (A) with gradient elution. The detection wavelength and column temperature were 253 nm and 40 ℃, respectively. The flow rate was 1.0 mL · min-1. The injection volume was 10 μL. The chemical attribution of the fingerprint was determined by reference substance comparison method. RESULTS The HPLC-UV fingerprints of 10 batches of M. firmum were calibrated with 21 common peaks. Among them, three flavone C-glycosides were identified. The similarities of the chromatograms of 10 batches of M. firmum were greater than 0.95. CONCLUSION The method is simple and reliable, which has satisfactory precision, repeatability and stability. It can be used for the quality control of M. firmum.
关键词
朝药材 /
肾炎草 /
高效液相色谱法 /
指纹图谱 /
黄酮碳苷
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Key words
Korean Chinese folk medicine /
Melandrium firmum Rohrb. /
HPLC /
fingerprint /
flavone C-glycoside
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金地区项目资助(81660646);吉林省中医药管理局科技项目资助(2015ZT52)
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